Systematic bioinformatics and experimental validation of yeast complexes reduces the rate of attrition during structural investigations.
نویسندگان
چکیده
For high-throughput structural studies of protein complexes of composition inferred from proteomics data, it is crucial that candidate complexes are selected accurately. Herein, we exemplify a procedure that combines a bioinformatics tool for complex selection with in vivo validation, to deliver structural results in a medium-throughout manner. We have selected a set of 20 yeast complexes, which were predicted to be feasible by either an automated bioinformatics algorithm, by manual inspection of primary data, or by literature searches. These complexes were validated with two straightforward and efficient biochemical assays, and heterologous expression technologies of complex components were then used to produce the complexes to assess their feasibility experimentally. Approximately one-half of the selected complexes were useful for structural studies, and we detail one particular success story. Our results underscore the importance of accurate target selection and validation in avoiding transient, unstable, or simply nonexistent complexes from the outset.
منابع مشابه
Surface-grafted Europium and Erbium Complexes of the 12-Tungstosilicate Heteropolyoxometalate: A Synthetic and Structural Investigations
The hydrothermal synthesis of two new inorganic-organic hybrid materials in which PDA (PDA = 1,10-phenanthroline-2,9-dicarboxylate) is connected to a well-defined Keggin-type polyoxometalate anion (POM) and a Ln(III) cation (POM = [SiW12O40]4−; Ln = Eu3+ (1), Er3+ (2)), are reported. These hybrids are characterized by elemental analyses, IR spectra and single-crystal X-ray diffraction. Single-c...
متن کاملPsychometric Properties of Breastfeeding Attrition Prediction Tool (BAPT): A Systematic Review
Background It is necessary to identify women who discontinue the breastfeeding to achieve the purpose of increasing the length of breastfeeding. To this end, Breastfeeding Attrition Prediction Tool (BAPT) has been developed. The current systematic review aimed to comprehensively review the validity and reliability of BAPT scale with different versions to give comprehensive information for autho...
متن کاملNumerical and Experimental Investigations on Springback of U-bending of DP600 Steel Alloy Sheet
The most prominent feature of sheet material forming process is an elastic recovery phenomenon during unloading which leads to springback and side wall curl. Therefore evaluation of springback and side wall curl is mandatory for production of precise products. In this paper, the effects of some parameters such as friction coefficient, sheet thickness, yield strength of sheet and blank-holder fo...
متن کاملCharacteristics of Different Brewerâs Yeast Strains Used for Non-alcoholic Beverage Fermentation in Media Containing Different Fermentable Sugars
Fermentation characteristics of four strains of brewer's yeast (Saccharomyces cerevisiae strain 70424, S.rouxii strain 2535, S. rouxii strain 2531 and Saccharomyces ludwigii strain 3447) in Yeast Moldbrothcontaining four different fermentable sugars (glucose, fructose, maltose, or sucrose) were studied. Theaim was to consider the suitability of different strain/sugar treatment...
متن کاملInvestigating the effect of structural form of outdoor path of residential complexes on improving the routing of people in emergency situations
Background and Objective: Due to the importance of time in choosing the optimal routing to reduce the damage caused by accidents in critical situations such as earthquakes, fires, etc., the structural form of the route has a significant impact on the selection of people in emergencies. It can also be very effective in the work for designers, managers and urban planners for disaster risk managem...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Structure
دوره 18 9 شماره
صفحات -
تاریخ انتشار 2010